Krenn L, Pötsch V
Department of Pharmacognosy, University of Vienna, Austria.
Pharmazie. 2006 Jul;61(7):582-5.
Food supplements containing soy extracts are increasingly used to treat menopausal complaints. For the quality control of such products a RP-HPLC method for the quantification of daidzein, genistein, glycitein and their respective glucosides and acetylglucosides in soy extracts and nutraceuticals was developed and validated. The extraction of the samples is very simple and avoids time-consuming handling and expensive cartridges or reagents. The compounds are quantified by internal standardization with 4'-hydroxyflavanon which is easily available at a reasonable price. On a base-deactivated C18 column good separation and excellent peak shape of the analytes are achieved in short time by gradient elution with water and acetonitrile. Accompanying substances do not interfere. The method was applied to the analysis of different commercial soy preparations.
含有大豆提取物的食品补充剂越来越多地用于治疗更年期不适。为了对这类产品进行质量控制，开发并验证了一种反相高效液相色谱法，用于定量大豆提取物和营养保健品中的大豆苷元、染料木黄酮、黄豆黄素及其各自的糖苷和乙酰葡糖苷。样品的提取非常简单，避免了耗时的操作以及昂贵的柱管或试剂。通过使用价格合理且易于获得的4'-羟基黄烷酮进行内标法定量这些化合物。在碱钝化的C18柱上，通过用水和乙腈进行梯度洗脱，可在短时间内实现分析物的良好分离和出色的峰形。共存物质不会产生干扰。该方法已应用于不同商业大豆制剂的分析。
