Kuhn R, Pravtcheva D, Ruddle F, Lemke G
Molecular Neurobiology Laboratory, Salk Institute, San Diego, California 92138.
J Neurosci. 1990 Jan;10(1):205-9. doi: 10.1523/JNEUROSCI.10-01-00205.1990.
We have used somatic cell hybrids to map the gene encoding protein zero (P0), the major structural protein of peripheral myelin. Analysis of Southern blots of DNA obtained from these hybrids allows us to unambiguously assign the P0 gene to mouse chromosome 1. This observation indicates that mutations in the P0 gene do not account for Trembler, a chromosome 11 mutation that specifically affects myelination in the peripheral nervous system.
我们利用体细胞杂交技术来定位编码外周髓鞘主要结构蛋白零（P0）的基因。对从这些杂交细胞获得的DNA进行Southern印迹分析，使我们能够明确地将P0基因定位于小鼠的1号染色体上。这一观察结果表明，P0基因的突变并不能解释震颤鼠（Trembler）的症状，震颤鼠是一种11号染色体突变体，其特异性地影响外周神经系统的髓鞘形成。
