You K H, Hsieh C L, Hayes C, Stahl N, Francke U, Popko B
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599.
Genomics. 1991 Apr;9(4):751-7. doi: 10.1016/0888-7543(91)90370-t.
We have cloned and characterized the mouse gene, P0, that encodes the predominant protein of peripheral myelin. Similar to the rat gene, the mouse P0 gene is encoded by six exons that span about 7 kb of DNA. The DNA sequence of the mouse gene is highly homologous with the rat gene, including the regions believed to be important in transcriptional control. Furthermore, the P0 protein appears well conserved throughout evolution. The gene was mapped to mouse chromosome 1 by Southern analysis of a Chinese hamster x mouse somatic cell hybrid panel. Several polymorphic restriction enzyme sites were identified within the P0 locus. Recombinant inbred strain mapping has linked the P0 gene to Ly-9/Ly/Sap in a region corresponding to band 1H3.
我们已经克隆并鉴定了编码外周髓鞘主要蛋白的小鼠基因P0。与大鼠基因相似，小鼠P0基因由六个外显子编码，跨越约7kb的DNA。小鼠基因的DNA序列与大鼠基因高度同源，包括被认为在转录控制中很重要的区域。此外，P0蛋白在整个进化过程中似乎都得到了很好的保守。通过对中国仓鼠×小鼠体细胞杂交细胞系进行Southern分析，该基因被定位到小鼠1号染色体上。在P0基因座内鉴定出了几个多态性限制酶切位点。重组近交系定位已将P0基因与Ly-9/Ly/Sap连锁在对应于1H3带的区域。
