Sripalwit Pralongyut, Wongsawad Chalobol, Wongsawad Pheravut, Anuntalabhochai Somboon
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand.
Exp Parasitol. 2007 Jan;115(1):98-102. doi: 10.1016/j.exppara.2006.05.005. Epub 2006 Aug 4.
The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers.
对来自清迈府和南奔府的37头印度瘤牛的瘤胃吸虫进行了调查，总体感染率为78.38%（29/37）。发现了三种吸虫：表层双口吸虫、链状正腔吸虫和长形菲策吸虫，感染率分别为75.68%、48.65%和40.54%。基于高退火温度随机扩增多态性DNA（HAT-RAPD）技术，通过聚合酶链反应扩增基因组DNA。5种随机10聚体寡核苷酸引物（OPA2、OPA4、OPB18、OPC9和OPH11）在这三种吸虫中产生了明显的条带模式。使用10种随机引物未鉴定出这三种吸虫的遗传变异。
