Phalee Anawat, Wongsawad Chalobol
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Applied Technology in Biodiversity Research Unit, Institute of Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Applied Technology in Biodiversity Research Unit, Institute of Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
Asian Pac J Trop Med. 2014 Mar;7(3):207-11. doi: 10.1016/S1995-7645(14)60022-5.
To investigate the infection of Fasciola gigantica (F. gigantica) in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.
The liver and gall bladder of Bubalus bubalis (B. bubalis) and Bos taurus (B. taurus) from slaughterhouses were examined adult worms and prevalence investigation. The species confirmation with phylogenetic analysis using ITS-2 sequences was performed by maximum likelihood and UPGMA methods.
The total prevalences of infection in B. bubalis and Bubalus taurus (B. taurus) were 67.27% and 52.94% respectively. The respective prevalence in both B. bubalis and B. taurus were acquired from Doi-Saket, Muang, and Sanpatong districts, with 81.25%, 62.50% and 60.00% for B. bubalis and 62.50%, 50.00% and 47.06% for Bos taurus respectively. The species confirmation of F. gigantica and some related species by basing on maximum likelihood and UPGMA methods used, 4 groups of trematodes were generated, first F. gigantica group including specimen of Chiang Mai, second 2 samples of F. hepatica, third group of 3 rumen flukes; Orthocoelium streptocoelium, F. elongatus and Paramphistomum epliclitum and fourth group of 3 minute intestinal flukes; Haplorchis taichui, Stellantchasmu falcatus, Haplorchoides sp. and liver fluke; Opisthorchis viverrini respectively.
These results can be confirmed the Giant liver fluke which mainly caused fascioliasis in Chiang Mai was identified as F. gigantica and specimens were the same as those of F. gigantica recorded in other different countries. Nucleotide sequence of ITS-2 region has been proven as effective diagnostic tool for the identification of F. gigantica.
调查清迈省家牛感染巨片形吸虫（Fasciola gigantica）的情况，并利用内转录间隔区2（ITS-2）区域进行分子鉴定。
对屠宰场的水牛（Bubalus bubalis）和黄牛（Bos taurus）的肝脏和胆囊进行检查，以发现成虫并进行患病率调查。使用最大似然法和非加权组平均法（UPGMA），通过ITS-2序列的系统发育分析进行物种鉴定。
水牛和黄牛的总感染率分别为67.27%和52.94%。水牛和黄牛的感染率分别来自多伊萨凯、孟和桑帕通地区，水牛的感染率分别为81.25%、62.50%和60.00%，黄牛的感染率分别为62.50%、50.00%和47.06%。基于最大似然法和UPGMA法对巨片形吸虫和一些相关物种进行物种鉴定，产生了4组吸虫，第一组为巨片形吸虫组，包括清迈的样本；第二组为2个肝片形吸虫样本；第三组为3种瘤胃吸虫，即链状正腔吸虫（Orthocoelium streptocoelium）、长形片形吸虫（F. elongatus）和显形双腔吸虫（Paramphistomum epliclitum）；第四组为3种微小肠吸虫，即泰国哈氏吸虫（Haplorchis taichui）、镰状星隙吸虫（Stellantchasmu falcatus）、类哈氏属吸虫（Haplorchoides sp.）和肝吸虫（Opisthorchis viverrini）。
这些结果可以证实，清迈地区主要引起片形吸虫病的巨肝吸虫被鉴定为巨片形吸虫，其样本与其他不同国家记录的巨片形吸虫样本相同。ITS-2区域的核苷酸序列已被证明是鉴定巨片形吸虫的有效诊断工具。
