1,3 - 丁二烯和二氧化氮及其光化学反应产物在果蝇和小鼠骨髓微核试验中的遗传毒性活性。

Genotoxic activity of 1,3-butadiene and nitrogen dioxide and their photochemical reaction products in Drosophila and in the mouse bone marrow micronucleus assay.

作者信息

Victorin K, Busk L, Cederberg H, Magnusson J

机构信息

Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

出版信息

Mutat Res. 1990 Feb;228(2):203-9. doi: 10.1016/0027-5107(90)90077-h.

DOI:10.1016/0027-5107(90)90077-h

PMID:1689006

Abstract

The genotoxic activity of a photochemical reaction mixture of 1,3-butadiene and nitrogen dioxide was investigated in vivo in the mouse bone marrow micronucleus assay and the somatic mutation and recombination test in Drosophila (the wing spot test). Butadiene alone was not mutagenic in Drosophila, but induced micronuclei in mice at 10 ppm after 23 h of exposure. Nitrogen dioxide was not genotoxic in either test system. The photochemical reaction products were toxic but probably not mutagenic in Drosophila and not genotoxic in mouse bone marrow. The in vivo results do not confirm earlier in vitro results that demonstrated a strong direct-acting mutagenic activity of the photochemical products in Salmonella.

摘要

在小鼠骨髓微核试验以及果蝇的体细胞突变和重组试验（翅斑试验）中，对1,3 - 丁二烯与二氧化氮的光化学反应混合物的遗传毒性活性进行了体内研究。单独的丁二烯在果蝇中不具有致突变性，但在小鼠中，暴露23小时后，浓度为10 ppm时可诱导产生微核。二氧化氮在这两种试验系统中均无遗传毒性。光化学反应产物具有毒性，但在果蝇中可能不具有致突变性，在小鼠骨髓中也无遗传毒性。体内试验结果并未证实早期的体外试验结果，早期体外试验表明光化学产物在沙门氏菌中具有很强的直接致突变活性。