滑子菇多糖对核糖核酸酶A的抑制动力学
Kinetics of inhibition of ribonuclease A by Pholiota Nameko polysaccharide.
作者信息
Li Haiping, Wang Shuo
机构信息
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, PR China.
出版信息
Int J Biol Macromol. 2007 Jan 30;40(2):134-8. doi: 10.1016/j.ijbiomac.2006.06.019. Epub 2006 Jun 29.
Pholiota nameko polysaccharide (PNPS-1) has been isolated and purified by enzyme hydrolysis, hot water extraction, ethanol precipitation, ion-exchange chromatography and gel-filtration column chromatography. The inhibition of bovine pancreas ribonuclease (RNase A) by PNPS-1 has been studied to elucidate the mechanism responsible for the decreased activity. PNPS-1 was effective in a linear mixed-type inhibition as suggested from the Lineweaver-Burk plot, Dixon plot and their replots. The values of K(i) and alphaK(i) were estimated as 299.92 and 545.71 microM, respectively. The alphaK(i) was greater than K(i) indicating that noncompetitive inhibition was predominant over competitive inhibition.
滑子菇多糖（PNPS - 1）已通过酶解、热水提取、乙醇沉淀、离子交换色谱和凝胶过滤柱色谱法进行分离和纯化。研究了PNPS - 1对牛胰腺核糖核酸酶（RNase A）的抑制作用，以阐明其活性降低的机制。根据Lineweaver - Burk图、Dixon图及其重绘图表明，PNPS - 1呈线性混合型抑制作用。K(i)和αK(i)值分别估计为299.92和545.71微摩尔。αK(i)大于K(i)，表明非竞争性抑制比竞争性抑制更占主导。
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