Petersen M J, Lessane B, Woodley D T
Department of Dermatology, University of North Carolina School of Medicine, Chapel Hill.
Arch Dermatol. 1990 Feb;126(2):175-80.
Biopsy specimens from unburned skin were obtained from three severely burned patients and placed into tissue culture. After 2 to 3 weeks, the cultured keratinocytes were released from the Petri dishes and transplanted onto the patient's burn wound, which had been completely excised down to muscle fascia, thereby removing all cutaneous elements. Healing cultured autografts were found to become repopulated with Langerhans cells within 3 to 6 weeks. A neodermis rich in fibronectin rapidly formed between the autografts and muscle fascia. However, using monoclonal antibodies to cytokeratins as markers of differentiation, we found that the autograft keratinocytes expressed an abnormal pattern of differentiation that was similar to the differentiation seen in hyperproliferative states such as psoriasis. In contrast, healed split-thickness graft donor sites and reepithelialized interstices of mesh grafts maintained the basal keratinocyte staining pattern of normal skin with the AE-1 monoclonal antibody.
从三名重度烧伤患者未烧伤的皮肤获取活检标本，并将其置于组织培养中。2至3周后，将培养的角质形成细胞从培养皿中分离出来，移植到患者已完全切除至肌肉筋膜的烧伤创面，从而去除所有皮肤成分。发现愈合的培养自体移植物在3至6周内重新充满朗格汉斯细胞。富含纤连蛋白的新真皮在自体移植物和肌肉筋膜之间迅速形成。然而，使用细胞角蛋白单克隆抗体作为分化标志物，我们发现自体移植物角质形成细胞表达了一种异常的分化模式，类似于银屑病等增殖过度状态下的分化情况。相比之下，愈合的断层皮片供皮区和网状皮片的再上皮化间隙用AE-1单克隆抗体维持正常皮肤的基底角质形成细胞染色模式。
