Schurman S H, Sharon N, Goldschmidt R A, Scanlon E F
Department of Surgery, Evanston Hospital, Ill 60201.
Arch Surg. 1990 Feb;125(2):179-82. doi: 10.1001/archsurg.1990.01410140057010.
We present a new method for detection of micrometastases to axillary lymph nodes and estrogen receptor determination. Cellular suspensions from primary infiltrating ductal breast carcinoma or level I axillary lymph nodes of patients who underwent mastectomies were obtained, by loosely grinding fresh tumors or lymph nodes through a grid and then transferring the matrix to a slide using cytocentrifugation. Tumor samples were analyzed for estrogen receptor status using an immunocytochemical kit and compared with the dextran-coated charcoal method. Thirty-eight of 48 correlated (20 were estrogen positive, and 18 were estrogen negative). Seven of 46 were estrogen positive while results from the dextran-coated charcoal method were estrogen negative. One of 46 was estrogen negative, while the results from the dextran-coated charcoal method were estrogen positive. Lymph node slide preparations were stained to detect tumor cells using antikeratin monoclonal antibodies. Three of 8 node-negative patients were found to have micrometastases. Four of 15 node-positive patients had additional nodes with tumor. Our method combines the advantages of serial sectioning and immunohistochemical staining.
我们提出了一种检测腋窝淋巴结微转移及雌激素受体测定的新方法。通过用网格松散研磨新鲜肿瘤或淋巴结，然后使用细胞离心涂片法将基质转移到载玻片上，获取接受乳房切除术患者的原发性浸润性导管乳腺癌或I级腋窝淋巴结的细胞悬液。使用免疫细胞化学试剂盒分析肿瘤样本的雌激素受体状态，并与葡聚糖包被活性炭法进行比较。48例中有38例结果相关（20例雌激素阳性，18例雌激素阴性）。46例中有7例雌激素阳性，而葡聚糖包被活性炭法结果为雌激素阴性。46例中有1例雌激素阴性，而葡聚糖包被活性炭法结果为雌激素阳性。使用抗角蛋白单克隆抗体对淋巴结载玻片标本进行染色以检测肿瘤细胞。8例淋巴结阴性患者中有3例被发现有微转移。15例淋巴结阳性患者中有4例其他淋巴结有肿瘤。我们的方法结合了连续切片和免疫组织化学染色的优点。
