Gonzalez-Arnao Maria Teresa, Engelmann Florent
Universidad Veracruzana, Facultad de Ciencias Químicas. Prol. OTE. 6, No. 1009, CP 94340, Apartado Postal 215, Orizaba, Veracruz, Mexico.
Cryo Letters. 2006 May-Jun;27(3):155-68.
Encapsulation-dehydration is a cryopreservation technique based on the technology developed for producing synthetic seeds, i.e. the encapsulation of explants in calcium alginate beads. Encapsulated explants are then precultured in liquid medium with a high sucrose concentration and partially desiccated before freezing. Encapsulating the explants allows the subsequent application of very drastic treatments including preculture with high sucrose concentrations and desiccation to low moisture contents which would be highly damaging or lethal to non-encapsulated samples. An encapsulation-dehydration protocol comprises the following steps: pretreatment, encapsulation, preculture, desiccation, freezing and storage, thawing and regrowth. Encapsulation-dehydration has been applied to around 40 different plant species. The optimization of the successive steps of the encapsulation-dehydration protocol is illustrated for sugarcane apices.
包埋脱水法是一种基于合成种子生产技术开发的冷冻保存技术，即将外植体包埋在海藻酸钙珠中。然后将包埋的外植体在高蔗糖浓度的液体培养基中进行预培养，并在冷冻前进行部分干燥。对外植体进行包埋可以使后续能够采用非常剧烈的处理方法，包括用高蔗糖浓度进行预培养以及干燥至低含水量，而这些处理对于未包埋的样品来说会具有高度的损伤性甚至是致死性。包埋脱水法方案包括以下步骤：预处理、包埋、预培养、干燥、冷冻与保存、解冻与再生长。包埋脱水法已应用于约40种不同的植物物种。文中以甘蔗茎尖为例说明了包埋脱水法方案连续步骤的优化。
