采用包埋脱水技术对柑橘胚珠和体细胞胚进行冷冻保存。
Cryopreservation of ovules and somatic embryos of citrus using the encapsulation-dehydration technique.
作者信息
González-Arnao M T, Juárez J, Ortega C, Navarro L, Duran-Vila N
机构信息
Facultad de Biología, Universidad de La Habana, Ciudad de La Habana, Cuba.
出版信息
Cryo Letters. 2003 Mar-Apr;24(2):85-94.
Cryopreservation of ovules and somatic embryos from several genotypes of citrus was achieved using the encapsulation-dehydration technique. Survival of cryopreserved ovules was occasional and erratic after different pregrowth conditions in liquid medium with 0.75M, 1M or up to 1.25M sucrose. An efficient cryopreservation protocol was established for somatic embryos derived from two embryogenic sources (ovules and cut thin layer explants from stigma, style and ovaries). High survival rates (75-100%) were consistently obtained after 1 day pregrowth in 0.75M sucrose, desiccation down to 20-25% moisture content in the beads and direct immersion in liquid nitrogen. The histological study showed that embryos subjected to the encapsulation-dehydration, accumulated high sucrose levels which appear to ensure the recovery of the whole embryo after cryopreservation.
采用包埋脱水技术实现了几种柑橘基因型胚珠和体细胞胚的冷冻保存。在含有0.75M、1M或高达1.25M蔗糖的液体培养基中，经过不同的预培养条件后，冷冻保存的胚珠存活率偶尔出现且不稳定。针对源自两种胚性来源（胚珠以及来自柱头、花柱和子房的切割薄层外植体）的体细胞胚，建立了一种高效的冷冻保存方案。在0.75M蔗糖中预培养1天、将珠子中的水分含量干燥至20-25%并直接浸入液氮后， consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently consistently 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Zotero 插件