Fushiki M, Ono K, Sasai K, Shibamoto Y, Tsutsui K, Nishidai T, Takahashi M, Abe M
Department of Radiology, Kyoto University Hospital, Japan.
Int J Radiat Oncol Biol Phys. 1990 Feb;18(2):353-7. doi: 10.1016/0360-3016(90)90100-x.
We report the effect of human granulocyte colony-stimulating factor (hG-CSF) on the recovery from granulocytopenia induced by irradiation. Female 9-week old C3H/He mice were used. The irradiation schedule was as follows: Group 1 and 2 received whole-body irradiation of 1 Gy and 5 Gy, respectively, on day 0; Group 3 and 4 received whole-body irradiation of 0.5 and 1.0 Gy, respectively, for 5 consecutive days; Group 5 received upper hemibody irradiation of 3 Gy for 5 consecutive days. Daily subcutaneous injections of G-CSF (3 x 10(5) Unit/mouse) or 0.3 ml of saline to each group were started from the day after the first irradiation and continued for 18 days. Mice were sampled randomly from each group, and the total number of leukocytes, erythrocytes of peripheral blood, nucleated cells in femur, and spleen weight were counted and measured, respectively, on day 0, 3, 5, 7, 9, 12, and 18. The leukocyte counts decreased with an increase in radiation doses. In Group 1 and 2 mice, G-CSF enhanced the leukocyte count more than saline. In Group 3 mice, the recovery of leukocytopenia was facilitated by G-CSF, but in Group 4 mice, G-CSF had no effect on the leukocyte count decrease or on leukocytopenia recovery. In Group 5 mice, G-CSF greatly affected leukocytopenia recovery. Increase in spleen weight paralleled the peripheral leukocyte count. Daily administration of recombinant hG-CSF accelerated the granulocytopenia recovery which was induced by irradiation, and it may be a useful therapeutic agent for treating myelosuppressive cases.
我们报告了人粒细胞集落刺激因子（hG-CSF）对辐射诱导的粒细胞减少症恢复的影响。使用9周龄雌性C3H/He小鼠。辐射方案如下：第1组和第2组分别在第0天接受1 Gy和5 Gy的全身照射；第3组和第4组分别连续5天接受0.5 Gy和1.0 Gy的全身照射；第5组连续5天接受3 Gy的上半身照射。从首次照射后的第二天开始，每天给每组皮下注射G-CSF（3×10⁵单位/只小鼠）或0.3 ml生理盐水，并持续18天。从每组中随机抽取小鼠，分别在第0、3、5、7、9、12和18天对白细胞总数、外周血红细胞、股骨中有核细胞以及脾脏重量进行计数和测量。白细胞计数随着辐射剂量的增加而降低。在第1组和第2组小鼠中，G-CSF比生理盐水更能提高白细胞计数。在第3组小鼠中，G-CSF促进了白细胞减少症的恢复，但在第4组小鼠中，G-CSF对白细胞计数的降低或白细胞减少症的恢复没有影响。在第5组小鼠中，G-CSF对白细胞减少症的恢复有很大影响。脾脏重量的增加与外周白细胞计数平行。每日给予重组hG-CSF可加速辐射诱导的粒细胞减少症的恢复，它可能是治疗骨髓抑制病例的一种有用的治疗药物。
