Sheikh F D, Bhattacharya T K, Kumar P, Sharma A
Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP 243 122, India.
Int J Immunogenet. 2006 Aug;33(4):271-6. doi: 10.1111/j.1744-313X.2006.00612.x.
PstI and TaqI restriction fragment length polymorphism (RFLP) of caprine DRB3.2 revealed three genotypes under each RFLP in Changthangi goat. The genotypic and allelic frequency in PstI RFLP was calculated as 0.07, 0.72 and 0.20 for PP, Pp and pp genotypes and 0.43 and 0.57 for P and p alleles. In TaqI RFLP the frequencies were observed as 0.11, 0.61 and 0.27 for TT, Tt and tt genotypes and 0.41, 0.59 for T and t alleles. Alignment study indicates variability of nucleotides and amino acids between alleles and different breeds of goats. The nucleotide sequences reported in this paper have been submitted to the European Molecular Biology Laboratory GenBank and the accession numbers were AY496935, AY496061 and AY496062. A significant affect (P <or= 0.05) of MHC DRB3.2 PstI polymorphism was found on the first and second year of pashmina production.
藏系山羊DRB3.2的PstI和TaqI限制性片段长度多态性（RFLP）显示，在每种RFLP下藏系山羊均有三种基因型。PstI RFLP中PP、Pp和pp基因型的基因型频率和等位基因频率分别计算为0.07、0.72和0.20，P和p等位基因的频率分别为0.43和0.57。在TaqI RFLP中，TT、Tt和tt基因型的频率分别为0.11、0.61和0.27，T和t等位基因的频率分别为0.41和0.59。比对研究表明等位基因之间以及不同品种山羊的核苷酸和氨基酸存在变异性。本文报道的核苷酸序列已提交至欧洲分子生物学实验室基因库，登录号分别为AY496935、AY496061和AY496062。发现MHC DRB3.2 PstI多态性对第一年和第二年的羊绒产量有显著影响（P≤0.05）。
