Pan C Y, Lan X Y, Zhao H Y, Hu S R, Huai Y T, Lei C Z, Chen H
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Genet Mol Res. 2011 Nov 22;10(4):3888-900. doi: 10.4238/2011.November.22.9.
We looked for novel genetic variations within the Six6 gene by PCR-SSCP, DNA sequencing and forced RFLP-PCR and estimated their associations with production traits in 2132 goats of eight indigenous Chinese breeds. A novel single nucleotide polymorphism (NM_001104993.1: g.232T>C) within the goat Six6 gene was identified. The frequencies of allele "C" varied from 0.8621 to 1.000, which were in Hardy-Weinberg equilibrium. Genotype and allele frequencies were found to be significantly different in the breeds. Association analysis revealed a significant relationship between genotypes and cannon circumference in Hainan black goats (P = 0.047); adult individuals with genotype CT showed higher cannon circumference than those with genotype CC. Another significant association of genotypes with five-year-old fiber length was found in Inner Mongolia white cashmere goats (P = 0.002). In addition, individuals with genotype CT had longer fiber length than those with genotype CC. The data revealed that the Six6 gene positively affects growth traits and cashmere traits. Polymorphism of Six6-PstI could be useful as a DNA marker for goat breeding and genetics via marker-assisted selection.
我们通过聚合酶链反应-单链构象多态性分析（PCR-SSCP）、DNA测序和强制限制性片段长度多态性聚合酶链反应（forced RFLP-PCR），在Six6基因中寻找新的基因变异，并评估它们与8个中国本土山羊品种的2132只山羊生产性状的关联。在山羊Six6基因中鉴定出一个新的单核苷酸多态性（NM_001104993.1: g.232T>C）。等位基因“C”的频率在0.8621至1.000之间，处于哈迪-温伯格平衡状态。发现该品种的基因型和等位基因频率存在显著差异。关联分析显示，海南黑山羊的基因型与管围之间存在显著关系（P = 0.047）；基因型为CT的成年个体管围高于基因型为CC的个体。在内蒙古白绒山羊中，发现基因型与5岁羊的纤维长度之间存在另一个显著关联（P = 0.002）。此外，基因型为CT的个体纤维长度长于基因型为CC的个体。数据表明，Six6基因对生长性状和羊绒性状有正向影响。Six6-PstI的多态性可作为一种DNA标记，通过标记辅助选择用于山羊育种和遗传学研究。
