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通过微阵列表达谱分析鉴定皮肤鳞状细胞癌中差异表达的基因。

Identification of differentially expressed genes in cutaneous squamous cell carcinoma by microarray expression profiling.

作者信息

Nindl Ingo, Dang Chantip, Forschner Tobias, Kuban Ralf J, Meyer Thomas, Sterry Wolfram, Stockfleth Eggert

机构信息

Department of Dermatology, Charité, Skin Cancer Center Charité, University Hospital of Berlin, Charitéplatz 1, D-10117 Berlin, Germany.

出版信息

Mol Cancer. 2006 Aug 8;5:30. doi: 10.1186/1476-4598-5-30.

DOI:10.1186/1476-4598-5-30
PMID:16893473
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1569867/
Abstract

BACKGROUND

Carcinogenesis is a multi-step process indicated by several genes up- or down-regulated during tumor progression. This study examined and identified differentially expressed genes in cutaneous squamous cell carcinoma (SCC).

RESULTS

Three different biopsies of 5 immunosuppressed organ-transplanted recipients each normal skin (all were pooled), actinic keratosis (AK) (two were pooled), and invasive SCC and additionally 5 normal skin tissues from immunocompetent patients were analyzed. Thus, total RNA of 15 specimens were used for hybridization with Affymetrix HG-U133A microarray technology containing 22,283 genes. Data analyses were performed by prediction analysis of microarrays using nearest shrunken centroids with the threshold 3.5 and ANOVA analysis was independently performed in order to identify differentially expressed genes (p < 0.05). Verification of 13 up- or down-regulated genes was performed by quantitative real-time reverse transcription (RT)-PCR and genes were additionally confirmed by sequencing. Broad coherent patterns in normal skin vs. AK and SCC were observed for 118 genes.

CONCLUSION

The majority of identified differentially expressed genes in cutaneous SCC were previously not described.

摘要

背景

肿瘤发生是一个多步骤过程，在肿瘤进展过程中有多个基因上调或下调。本研究检测并鉴定了皮肤鳞状细胞癌（SCC）中差异表达的基因。

结果

对5名免疫抑制器官移植受者的三种不同活检组织进行了分析，每种活检组织包括正常皮肤（全部合并）、光化性角化病（AK）（合并了两份）和浸润性SCC，此外还分析了5名免疫功能正常患者的正常皮肤组织。因此，使用15个样本的总RNA与包含22,283个基因的Affymetrix HG-U133A微阵列技术进行杂交。通过使用阈值为3.5的最近收缩质心的微阵列预测分析进行数据分析，并独立进行方差分析以鉴定差异表达的基因（p < 0.05）。通过定量实时逆转录（RT）-PCR对13个上调或下调的基因进行验证，并通过测序进一步确认这些基因。观察到118个基因在正常皮肤与AK和SCC之间呈现广泛的连贯模式。

结论

皮肤SCC中鉴定出的大多数差异表达基因此前未曾被描述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c9/1569867/615e7ec9b110/1476-4598-5-30-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c9/1569867/40e3ed35a477/1476-4598-5-30-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c9/1569867/615e7ec9b110/1476-4598-5-30-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c9/1569867/40e3ed35a477/1476-4598-5-30-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c9/1569867/615e7ec9b110/1476-4598-5-30-2.jpg

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