Kuerner Klaus-Michael, Steinbeisser Herbert
Institute of Human Genetics, University of Heidelberg, Heidelberg, Germany.
Dev Dyn. 2006 Oct;235(10):2861-7. doi: 10.1002/dvdy.20910.
To date, five members of the insulin-like growth factor-binding protein (IGFBP) superfamily have been described in Xenopus laevis. Here, we report the isolation of two new IGFBPs: xIGFBP-rP10, and xIGFBP-like. The proteins share the same domain architecture, and together with Mig30, form a subgroup within the IGFBP superfamily. Temporal expression analysis shows that they are expressed differentially during early development. xIGFBP-rP10 is continuously expressed, whereas Mig30 expression peaks during gastrulation. IGFBP-like is expressed from neurulation onward. The three genes have characteristic spatial expression domains, which overlap in some regions. Both xIGFBP-rP10 and Mig30 are expressed on the dorsal side of the embryo during gastrulation. Later, xIGFBP-rP10 is expressed in the notochord, the floor plate, the somites, and the fin. xIGFBP-like expression is seen primarily in the developing central nervous system and overlaps with Mig30 expression at the end of neurulation in the developing somites and in tail bud stages in the eyes.
迄今为止，在非洲爪蟾中已发现胰岛素样生长因子结合蛋白（IGFBP）超家族的五个成员。在此，我们报告两种新的IGFBP的分离：xIGFBP-rP10和xIGFBP样蛋白。这些蛋白具有相同的结构域架构，并与Mig30一起在IGFBP超家族中形成一个亚组。时序表达分析表明，它们在早期发育过程中差异表达。xIGFBP-rP10持续表达，而Mig30的表达在原肠胚形成期达到峰值。xIGFBP样蛋白从神经胚形成期开始表达。这三个基因具有特征性的空间表达结构域，在某些区域重叠。在原肠胚形成期，xIGFBP-rP10和Mig30均在胚胎的背侧表达。之后，xIGFBP-rP10在脊索、底板、体节和鳍中表达。xIGFBP样蛋白的表达主要见于发育中的中枢神经系统，并且在神经胚形成末期在发育中的体节以及眼的尾芽阶段与Mig30的表达重叠。
