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[法氏囊尾蚴(囊尾蚴科)碳水化合物代谢的酶]

[The enzymes of carbohydrates metabolism from Cysttidicola farionis (Cystidicolidae)].

作者信息

Zółtowska K, Lopieńska E, Rokicki J, Dmitryjuk M

机构信息

Zakład Biochemii, Wydział Biologii, UWM, 10-561 Olsztyn, ul. Zołnierska 14.

出版信息

Wiad Parazytol. 2001;47(3):311-5.

Abstract

The content of glycogen, glucose and trehalose was measured in larvae and adults of Cystidicola farionis, the parasite isolated from the swim bladder of Osmerus eperlanus from Vistula Lagoon. Activity of glycogen phosphorylase, alpha-amylase, glucoamylase, maltase, trehalase, and trehalose phosphorylase were measured. The highest activity was recorded for alpha-amylase 10.07 +/- 0.97 mu/mg and 7.47 +/- 0.24 mu/mg, next maltase 1.34 +/- 0.63 micromol/mg and 2.06 +/- 1.65 micronol/mg respectively for larvae and adults. The activity of glucoamylase was nearly the same for adults and larvae (about 0.20 micromol/mg). The trehalase activity was higher at adults (0.49 +/- 0.42 micromol/mg) than at larvae (0.18 +/- 0.12 micromol/mg). The activity of glycogen phosphorylase was much higher at larvae (3.58 +/- 1.49 micromol/mg) than at adults parasite (0.10 +/- 0.02 micromol/mg). The trehalose phosphorylase was present in both stages of parasite, but its activity was low. The content of glycogen and glucose was two-times higher in the adults' body than in larvae.

摘要

对从维斯瓦河泻湖的胡瓜鱼鱼鳔中分离出的寄生虫——法氏囊线虫的幼虫和成虫体内的糖原、葡萄糖和海藻糖含量进行了测定。同时测定了糖原磷酸化酶、α-淀粉酶、葡糖淀粉酶、麦芽糖酶、海藻糖酶和海藻糖磷酸化酶的活性。其中,α-淀粉酶活性最高,幼虫和成虫分别为10.07±0.97 μ/mg和7.47±0.24 μ/mg;其次是麦芽糖酶,幼虫和成虫分别为1.34±0.63 μmol/mg和2.06±1.65 μmol/mg。葡糖淀粉酶在成虫和幼虫中的活性几乎相同(约0.20 μmol/mg)。海藻糖酶活性在成虫中较高(0.49±0.42 μmol/mg),而在幼虫中较低(0.18±0.12 μmol/mg)。糖原磷酸化酶活性在幼虫中(3.58±1.49 μmol/mg)远高于成虫寄生虫(0.10±0.02 μmol/mg)。海藻糖磷酸化酶在寄生虫的两个阶段均有存在,但其活性较低。成虫体内的糖原和葡萄糖含量比幼虫高出两倍。

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