Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
Department of Medical Biology, School of Public Health, Collegium Medicum, University of Warmia and Mazury, 10-561 Olsztyn, Poland.
Int J Environ Res Public Health. 2022 May 25;19(11):6430. doi: 10.3390/ijerph19116430.
The molecular identification of (a swim bladder nematode of European smelt from the Vistula Lagoon in Poland) was performed. Their prevalence level was determined, and changes in the trehalose synthesis pathway in larvae and adult nematodes were demonstrated. The trehalose level was almost four times higher in adult nematodes than in larvae. In contrast, the activity of both enzymes (trehalose 6-phosphate synthase, TPS and trehalose 6-phosphate phosphatase, TPP) involved in the synthesis of trehalose was higher in larvae than in adults under optimal conditions. The optimum pH for TPS isolated from larvae and adults was pH 7.0. The optimum pH for TPP from larvae and adults was pH 7.0 and pH 8.0, respectively. The optimal temperature was 20 °C, and Mg ions were an activator for trehalose-synthetizing enzymes from both sources. Enzymes isolated from adult nematodes were less susceptible to divalent ion chelator and inorganic phosphate than larval enzymes. The dynamic transformation of trehalose in the nematode developing inside the swim bladder of the smelt appears to be an important metabolic pathway in the nematode survival strategy. These studies are aimed at a better understanding of the issue of the metabolic adaptation of parasites, which, in the future, may indirectly contribute to the elimination of the parasite from aquacultures, which will impact public health.
对（一种来自波兰维斯图拉泻湖的欧洲胡瓜鱼的鳔咽线虫）进行了分子鉴定。确定了它们的流行程度，并证明了幼虫和成虫线虫中海藻糖合成途径的变化。与幼虫相比，成虫线虫中的海藻糖水平几乎高出四倍。相比之下，在最佳条件下，参与海藻糖合成的两种酶（海藻糖 6-磷酸合酶，TPS 和海藻糖 6-磷酸磷酸酶，TPP）在幼虫中的活性均高于成虫。从幼虫和成虫中分离出的 TPS 的最适 pH 值为 7.0。幼虫和成虫来源的 TPP 的最适 pH 值分别为 7.0 和 8.0。最适温度为 20°C，Mg 离子是两种来源的海藻糖合成酶的激活剂。与幼虫来源的酶相比，来自成虫线虫的酶对二价离子螯合剂和无机磷酸盐的敏感性较低。在鳔中发育的线虫中海藻糖的动态变化似乎是线虫生存策略中的重要代谢途径。这些研究旨在更好地了解寄生虫代谢适应的问题，这可能会间接有助于从水产养殖中消除寄生虫，从而影响公共健康。
