Myjak P, Majewska A C, Bajer A, Siński E, Wedrychowicz H, Gołab E, Budak A, Stańczak J
Zakład Parazytologii Tropikalnej, Instytut Medycyny Morskiej i Tropikalnej, 81-519 Gdynia, ul. Powstania Styczniowego 9 b.
Wiad Parazytol. 2001;47(3):433-55.
After a long period of using basic microscopic, immunological and biochemical methods for diagnosis, rapid development of nucleic acids investigation enabled introduction of specific and sensitive methods of detection of pathogenic agents on the molecular level. Among others, polymerase chain reaction (PCR), discovered in mid of 80'ies and then automatized, offered an attractive alternative to conventional testing systems. In this paper we describe reliable diagnostic tests widely used in the world, including Poland, and capable of detecting different disease agents as parasites and fungi in clinical specimens and pathogens of emerging zoonotic diseases in ticks. The possibilities of using molecular methods for determination of Plasmodium falciparum drug resistance is also discussed. Moreover, the report offers information concerning kinds of molecular tests and institutions in which there are executed.
在长期使用基本的显微镜、免疫学和生物化学方法进行诊断之后，核酸研究的迅速发展使得在分子水平上引入特异性和灵敏的病原体检测方法成为可能。其中，20世纪80年代中期发现并随后实现自动化的聚合酶链反应（PCR）为传统检测系统提供了一种有吸引力的替代方法。在本文中，我们描述了在世界范围内（包括波兰）广泛使用的可靠诊断测试，这些测试能够检测临床标本中的寄生虫和真菌等不同病原体以及蜱中新兴人畜共患病的病原体。还讨论了使用分子方法测定恶性疟原虫耐药性的可能性。此外，该报告提供了有关分子检测种类以及进行这些检测的机构的信息。
