Ling M L
Department of Pathology, Singapore General Hospital.
Singapore Med J. 1994 Jun;35(3):316-8.
The application of monoclonal antibodies and DNA probes in the clinical microbiology laboratory has resulted in an array of rapid diagnostic tests. The immunofluorescent assay or enzyme-linked immunoassay is widely used in the rapid diagnosis of bacteria eg Group A streptococcus, Legionella pneumophila, Mycoplasma pneumoniae, Bordetella pertussis; parasites eg Chlamydia tachomatis, Cryptosporidium species; and fungi eg Pneumocystis carinii. The BACTEC system was first introduced to detect bacteraemia pathogens. It has been further developed to detect Mycobacterium species in clinical specimens and this has greatly reduced turn-around time in the laboratory diagnosis of Mycobacterium species. The discovery of the polymerase chain reaction has led to hopes of using it as a potential diagnostic tool in the microbiology laboratory.
单克隆抗体和DNA探针在临床微生物学实验室中的应用带来了一系列快速诊断测试。免疫荧光测定法或酶联免疫测定法广泛用于细菌的快速诊断，如A组链球菌、嗜肺军团菌、肺炎支原体、百日咳博德特氏菌；寄生虫，如沙眼衣原体、隐孢子虫属；以及真菌，如卡氏肺孢子虫。BACTEC系统最初用于检测菌血症病原体。它已进一步发展用于检测临床标本中的分枝杆菌属，这大大缩短了分枝杆菌属实验室诊断的周转时间。聚合酶链反应的发现带来了将其用作微生物学实验室潜在诊断工具的希望。
