Cencek T, Ziomko I, Karamon J
Zakład Parazytologii i Chorób Inwazyjnych, Państwowy Instytut Weterynaryjny 24-100 Puławy, ul. Partyzantów 57.
Wiad Parazytol. 2001;47(3):511-20.
The aim of investigations was to determine the stability of ELISA components preserved under different conditions. The following components for the ELISA were used: the secreted/excreted antigen prepared from H. bovis L1 larvae, its fraction containing hypodermine A, conjugat, positive and negative control sera. Freezing at -20 degrees C, lyophilization and preservation at 4 degrees C were applied. Seven kits for the ELISA containing components preserved using described methods, were prepared. The identical packets of sera were examined by the ELISA directly after preparation of the kits (day '0'), and next, at 3-4 week intervals for 9 months. The experiments showed that the best method of conservation of ELISA components for the detection of H. bovis antibodies was lyophilization. The kits prepared in this way did not lose their characteristics for 9 months and their specificity and sensitivity were close 100%.
研究目的是确定在不同条件下保存的酶联免疫吸附测定（ELISA）组件的稳定性。ELISA使用了以下组件：由牛副丝虫L1幼虫制备的分泌/排泄抗原、其含皮下碱A的组分、结合物、阳性和阴性对照血清。采用在-20℃冷冻、冻干以及在4℃保存的方法。制备了7个采用上述方法保存组件的ELISA试剂盒。试剂盒制备完成后（第0天）立即通过ELISA检测相同批次的血清样本，随后在9个月内每隔3 - 4周检测一次。实验表明，用于检测牛副丝虫抗体的ELISA组件的最佳保存方法是冻干。以这种方式制备的试剂盒在9个月内未丧失其特性，其特异性和敏感性接近100%。
