Ciarkowska J, Spausta G, Wiczkowski A, Adamek B, Beniowski M
Katedra i Zakład Ogólnej Biologii Lekarskiej Slaskiej Akademii Medycznej, 41-808 Zabrze, ul. Jordana 19.
Wiad Parazytol. 2001;47(3):543-8.
The frequency of Pneumocystis carinii occurrence in BAL of 38 HIV-infected patients was determined with three different method. BAL sediments were stained with Giemsa method, silvered according to Gomori-Grocott method and studied with indirect immunofluorescence assay. Using Giemsa method staining Pneumocystis carinii was diagnosed in 81.6% of patients, in Gomori-Grocott method--in 31.6% of patients, but results of indirect immunofluorescence assay were positive only in 23.,7%. In our study staining BAL sediments with Giemsa method allowed to detect Pneumocystis carinii in the highest percentage of examined patients.
采用三种不同方法测定了38例HIV感染患者支气管肺泡灌洗（BAL）液中卡氏肺孢子虫的检出率。BAL沉淀物用吉姆萨方法染色，根据戈莫里-格罗科特方法进行银染，并采用间接免疫荧光试验进行检测。使用吉姆萨方法染色，81.6%的患者被诊断出卡氏肺孢子虫；采用戈莫里-格罗科特方法，31.6%的患者被诊断出；但间接免疫荧光试验结果仅23.7%呈阳性。在我们的研究中，用吉姆萨方法对BAL沉淀物进行染色，在所检查患者中检测到卡氏肺孢子虫的比例最高。
