Gori S, Paladini A, Paoletti P, Del Bono L, Scasso A
Laboratorio di malattie infettive dell'Ospedale Cisanello di Pisa.
Pathologica. 1992 Mar-Apr;84(1090):225-33.
Authors review several methods for diagnosis of Pneumocystis carinii on BAL fluid in AIDS patients aiming to identify ideal routine technic. Giemsa, Gomori and Toluidine Blue staining. Direct and Indirect Immunofluorescence. Immunocytochemical methods were tested and advantages/disadvantages compared on BAL fluid from AIDS patients. Pneumocystis carinii was detected in 7 out 21 cases (33%). Gomori staining and Toluidine Blue staining were chosen for routine Pneumocystis carinii detection while IF technics are deserved as very useful in fields showing high fungal ++ contamination (inducted sputum).
作者回顾了几种用于诊断艾滋病患者支气管肺泡灌洗（BAL）液中卡氏肺孢子虫的方法，旨在确定理想的常规技术。对吉姆萨染色、戈莫里染色和甲苯胺蓝染色、直接和间接免疫荧光法、免疫细胞化学方法进行了测试，并比较了它们在艾滋病患者BAL液中的优缺点。21例中有7例（33%）检测到卡氏肺孢子虫。戈莫里染色和甲苯胺蓝染色被选用于常规卡氏肺孢子虫检测，而免疫荧光技术在真菌污染严重（诱导痰）的领域被认为非常有用。
