卡氏肺孢子虫的实验室诊断：细胞学、免疫荧光和免疫细胞化学染色的比较

[Laboratory diagnosis of Pneumocystis carinii: comparison of cytological, immunofluorescent and immunocytochemical staining].

作者信息

Gori S, Paladini A, Paoletti P, Del Bono L, Scasso A

机构信息

Laboratorio di malattie infettive dell'Ospedale Cisanello di Pisa.

出版信息

Pathologica. 1992 Mar-Apr;84(1090):225-33.

Abstract

Authors review several methods for diagnosis of Pneumocystis carinii on BAL fluid in AIDS patients aiming to identify ideal routine technic. Giemsa, Gomori and Toluidine Blue staining. Direct and Indirect Immunofluorescence. Immunocytochemical methods were tested and advantages/disadvantages compared on BAL fluid from AIDS patients. Pneumocystis carinii was detected in 7 out 21 cases (33%). Gomori staining and Toluidine Blue staining were chosen for routine Pneumocystis carinii detection while IF technics are deserved as very useful in fields showing high fungal ++ contamination (inducted sputum).

摘要

作者回顾了几种用于诊断艾滋病患者支气管肺泡灌洗（BAL）液中卡氏肺孢子虫的方法，旨在确定理想的常规技术。对吉姆萨染色、戈莫里染色和甲苯胺蓝染色、直接和间接免疫荧光法、免疫细胞化学方法进行了测试，并比较了它们在艾滋病患者BAL液中的优缺点。21例中有7例（33%）检测到卡氏肺孢子虫。戈莫里染色和甲苯胺蓝染色被选用于常规卡氏肺孢子虫检测，而免疫荧光技术在真菌污染严重（诱导痰）的领域被认为非常有用。

相似文献

1

[Laboratory diagnosis of Pneumocystis carinii: comparison of cytological, immunofluorescent and immunocytochemical staining].卡氏肺孢子虫的实验室诊断：细胞学、免疫荧光和免疫细胞化学染色的比较

Pathologica. 1992 Mar-Apr;84(1090):225-33.

2

Laboratory diagnosis and occurrence of Pneumocystis carinii.卡氏肺孢子虫的实验室诊断与发病情况

Scand J Infect Dis Suppl. 1994;94:1-34.

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[Pneumocystis carinii pneumonia: laboratory diagnosis].卡氏肺孢子虫肺炎：实验室诊断

Rev Argent Microbiol. 1992 Apr-Jun;24(2):53-9.

4

Use of a monoclonal antibody to detect Pneumocystis carinii in induced sputum and bronchoalveolar lavage fluid by immunoperoxidase staining.使用单克隆抗体通过免疫过氧化物酶染色法检测诱导痰和支气管肺泡灌洗液中的卡氏肺孢子虫。

Arch Pathol Lab Med. 1988 Dec;112(12):1233-6.

5

Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum with use of monoclonal antibodies.卡氏肺孢子虫肺炎的诊断：使用单克隆抗体提高痰液中的检测率。

N Engl J Med. 1988 Mar 10;318(10):589-93. doi: 10.1056/NEJM198803103181001.

6

Comparison of methods for identification of Pneumocystis carinii in bronchoalveolar lavage fluid.支气管肺泡灌洗 fluid 中卡氏肺孢子虫鉴定方法的比较。注：原英文文本中“bronchoalveolar lavage fluid”准确表述应该是“bronchoalveolar lavage fluid”，意为“支气管肺泡灌洗液体” ，这里原英文单词拼写有误，翻译时保留了错误拼写。正常翻译应该是“支气管肺泡灌洗术中获取的液体” ，可根据具体语境调整译文表述为“支气管肺泡灌洗术获取液” 等更准确的说法。

J Med Assoc Thai. 2002 Jun;85 Suppl 1:S407-14.

7

[Comparative study of 2 classical technics of coloration and an indirect immunofluorescent assay applied to research on Pneumocystis carinii in the bronchoalveolar lavage fluid and induced sputum in HIV+ patients].[两种经典染色技术与间接免疫荧光测定法在HIV阳性患者支气管肺泡灌洗液和诱导痰中卡氏肺孢子虫研究中的比较]

Ann Biol Clin (Paris). 1989;47(10):635-9.

8

Modification of the fungi-fluor and the genetic systems fluorescent antibody methods for detection of Pneumocystis carinii in bronchoalveolar lavage specimens.真菌荧光法及基因系统荧光抗体法的改良，用于检测支气管肺泡灌洗标本中的卡氏肺孢子虫。

Arch Pathol Lab Med. 1995 Feb;119(2):142-7.

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[Induced sputum. Indication for examination in Pneumocystis carinii pneumonia?].[诱导痰。卡氏肺孢子虫肺炎的检查指征？]

Tidsskr Nor Laegeforen. 1995 Feb 10;115(4):476-8.

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[The estimation of Pneumocystis carinii in HIV-infected patients occurrence based on three different methods].[基于三种不同方法对HIV感染患者中卡氏肺孢子虫感染发生率的估计]

Wiad Parazytol. 2001;47(3):543-8.

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