Akasaka Kazuaki, Ohtaki Takashi, Ohrui Hiroshi
Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
J Sep Sci. 2006 Jul;29(10):1390-8. doi: 10.1002/jssc.200600025.
Enantiomeric discrimination of chiral primary amines was performed by both reversed-phase HPLC and normal-phase HPLC after labeling with a chiral fluorescent derivatization reagent, (1R,2R)- and (1S,2S)-trans-2-(2,3-anthracenedicarboximido)cyclohexanecarbonyl chloride. Use of HPLC permits separation of diastereomeric derivatives of amines up to C30 which have a primary amino group at the middle of the alkyl chain. The derivatives of primary amines having an anteiso alkyl chain, which has a chiral branched-methyl at the n-3 position of the alkyl chain, were also separated by HPLC, and it was also possible to separate niphatesine D by reversed-phase HPLC after derivatization.
在手性荧光衍生化试剂(1R,2R)-和(1S,2S)-反式-2-(2,3-蒽二甲酰亚胺)环己烷甲酰氯标记后，通过反相高效液相色谱法和正相高效液相色谱法对手性伯胺进行对映体鉴别。高效液相色谱法的使用允许分离碳原子数高达C30且在烷基链中间具有伯氨基的胺的非对映体衍生物。具有前异烷基链（在烷基链的n-3位具有手性支链甲基）的伯胺衍生物也通过高效液相色谱法分离，并且在衍生化后通过反相高效液相色谱法也可以分离尼法西定D。
