Imaizumi Keiichirou, Terasima Hanae, Akasaka Kazuaki, Ohrui Hiroshi
Graduate School of Life Sciences, Tohoku University, Sendai 981-8555, Japan.
Anal Sci. 2003 Sep;19(9):1243-9. doi: 10.2116/analsci.19.1243.
Highly sensitive chiral labeling reagents, (1R,2R)- and (1S,2S)-2-(2,3-anthracenedicarboximido)cyclohexanecarboxylic acids [(1R,2R)- and (1S,2S)-A] and (1R,2R)- and (1S,2S)-2-(2,3-naphthalenedicarboximido)cyclohexanecarboxylic acids [(1R,2R)- and (1S,2S)-A'], were prepared. Reagent A has enabled us to discriminate the enantiomers of anteiso fatty alcohols up to C9 methyl branching by 1H NMR, and both reagents A and A' have allowed up to C16 methyl branching at the 10(-15) molar level by fluorescence detected reversed-phase HPLC.
制备了高灵敏度的手性标记试剂，即(1R,2R)-和(1S,2S)-2-(2,3-蒽二甲酰亚胺基)环己烷羧酸[(1R,2R)-和(1S,2S)-A]以及(1R,2R)-和(1S,2S)-2-(2,3-萘二甲酰亚胺基)环己烷羧酸[(1R,2R)-和(1S,2S)-A']。试剂A使我们能够通过1H NMR区分碳数达C9甲基支链的反异脂肪酸醇的对映体，并且试剂A和A'都能通过荧光检测反相高效液相色谱在10(-15)摩尔水平区分碳数达C16甲基支链的情况。
