Akasaka Kazuaki, Ohrui Hiroshi
Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem. 2004 Jan;68(1):153-8. doi: 10.1271/bbb.68.153.
Anteiso fatty acids having 16 to 29 carbon atoms were labeled with the chiral fluorescent conversion reagents, (1R,2R)- and (1S,2S)-2-(2,3-anthracenedicarboximido)cyclohexanol. The diastereomeric esters of anteiso acids having up to 20 carbon atoms were separated into two peaks in an ODS column under low column-temperature conditions, while those having more than 21 carbon atoms were not separated. A C30 column made it possible to separate diastereomeric esters up to C29 anteiso acid. It was possible to predict the absolute configuration of each acid by the elution order of the derivatives.
含有16至29个碳原子的反异脂肪酸用手性荧光转化试剂（1R,2R）-和（1S,2S）-2-（2,3-蒽二甲酰亚胺）环己醇进行标记。在低柱温条件下，含有20个碳原子以下的反异酸的非对映体酯在ODS柱上被分离为两个峰，而含有21个碳原子以上的则未被分离。C30柱能够分离出直至C29反异酸的非对映体酯。通过衍生物的洗脱顺序可以预测每种酸的绝对构型。
