Zhao Yu-gang, Zhou Ji, Zhang Xue-jun, Men Xiao-wei, Wang Rui, Zhou Fei, Chen De-hong, Yu Zhi-yun, Shi Hong-bo
Department of Urology, Xiangfan Central Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Xiangfan, Hubei 441021, China.
Zhonghua Nan Ke Xue. 2006 Jul;12(7):619-21.
To study the relationships of experimental varicocele to the apoptosis of epididymis epithelium and changes of the contents of alpha-1,4-glucosidase and sialic acid from the unilateral epididymis in adolescent rats, and to investigate the effects of varicocele on the unilateral epididymis epithelium.
Experimental left varicocele models of 16 adult male Sprague-Dawley rats were obtained by partial ligation of the left renal vein. The epididymides were collected for detecting the apoptosis of epididymis epithelium and the contents of alpha-1,4-glucosidase and sialic acid by using spectrophotometry.
Seven days after the establishment of the left varicocele model, the index of the apoptosis of the left epididymis epithelium was significantly higher (P < 0.001) and the contents of alpha-1,4-glucosidase and sialic acid significantly lower (P < 0.05, P < 0.005) in the experimental group than in the control.
The results suggest that unilateral varicocele may increase the apoptosis of epididymis epithelium and the contents of alpha-1,4-glucosidase and sialic acid and subsequently affect the synthesizing and secretory function of the epididymis.
研究青春期大鼠实验性精索静脉曲张与附睾上皮细胞凋亡及单侧附睾中α-1,4-葡萄糖苷酶和唾液酸含量变化的关系，并探讨精索静脉曲张对单侧附睾上皮的影响。
通过部分结扎左肾静脉建立16只成年雄性Sprague-Dawley大鼠的实验性左侧精索静脉曲张模型。收集附睾，采用分光光度法检测附睾上皮细胞凋亡及α-1,4-葡萄糖苷酶和唾液酸含量。
左侧精索静脉曲张模型建立7天后，实验组左侧附睾上皮细胞凋亡指数显著升高（P < 0.001），α-1,4-葡萄糖苷酶和唾液酸含量显著降低（P < 0.05，P < 0.005）。
结果表明单侧精索静脉曲张可能增加附睾上皮细胞凋亡及α-1,4-葡萄糖苷酶和唾液酸含量，进而影响附睾的合成和分泌功能。
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