[精索静脉曲张对青春期大鼠生精细胞凋亡相关基因Bcl-2和Bax表达的影响]
[Effect of varicocele on expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats].
作者信息
He Xue-jun, Chen Le-zhong, Fang De-xin, Ye He-song, She Shao-yi, Hong Han-ye
机构信息
Department of Urology, The First Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Jul;36(4):497-9, 518.
OBJECTIVE
To study the effect of varicocel (VC) on expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats, and investigate the mechanism of the infertility resulting from varicocele.
METHODS
The varicocele model was created by partial ligation of the left renal vein in the adolescent rats, two and four and eight weeks after creation of the varicocele model, the expressions of Bcl-2 and Bax were detected by immunohistochemical method.
RESULTS
With the duration of varicocele model, the Bcl-2 expression in experiment group was decreased as compared with that in control group. And in experiment group, the Bcl-2 expression level was lower in left testis than in right testis. But Bax expression in experiment group was increased as compared with that in control group, and in experiment group, the Bax expression level was higher in left testis than in right testis.
CONCLUSION
Experimental varicocele could change obviously expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats.
目的
研究精索静脉曲张（VC）对青春期大鼠生精细胞凋亡相关基因Bcl-2和Bax表达的影响，探讨精索静脉曲张导致不育的机制。
方法
通过部分结扎青春期大鼠左肾静脉建立精索静脉曲张模型，在建立模型后2周、4周和8周，采用免疫组织化学方法检测Bcl-2和Bax的表达。
结果
随着精索静脉曲张模型时间的延长，实验组Bcl-2表达较对照组降低。且在实验组中，左侧睾丸Bcl-2表达水平低于右侧睾丸。但实验组Bax表达较对照组增加，且在实验组中，左侧睾丸Bax表达水平高于右侧睾丸。
结论
实验性精索静脉曲张可明显改变青春期大鼠生精细胞凋亡相关基因Bcl-2和Bax的表达。
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