Zheng Yi-Qun, Zhang Xiao-Bin, Cheng Fan, Zhou Ben-Zheng, Rao Ting, Fei An-Hua
Department of Urology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, China.
Zhonghua Nan Ke Xue. 2008 Sep;14(9):805-9.
To investigate the impact of experimental varicocele (EV) on the ipsilateral testis in rats.
EV was induced by partial ligation of the left renal vein in male SD rats, the control rats subjected to sham operation, and the testes of the EV models and controls were extirpated 6, 12, and 18 weeks later. Johnson's score, ultrastructure of seminiferous tubules, intratesticular testosterone concentration (ITC) and germ cell apoptotic index (AI) of each left testis were evaluated.
Johnson's scores were (6.92 +/- 0.52), (4.83 +/- 0.41) and (2.95 +/- 0.26), ITCswere (6.32 +/- 0.85), (5.17 +/- 0.76) and (4.11 +/- 0.69) and AIs were (5.32 +/- 1.23), (15.21 +/- 0.97) and (21.13 +/- 1.12) respectively in the 6 w , 12 w and 18 w EV groups, significantly lower than in the corresponding control groups, (9.56 +/- 0.35, 9.63 +/- 0.31, 9.39 +/- 0.46), (9.64 +/- 1.23, 9.38 +/- 0.69, 9.73 +/- 0.49) and (3.21 +/- 1.15, 3.43 +/- 1.21, 3.61 +/- 1. 15) (P < 0.05), the former two showing a gradual decline while the latter a significant elevation with the increasing duration of varicocele. The damage to the ultrastructure of seminiferous tubules was aggravated with the prolonging of varicocele.
EV can cause a progressive decline of ITC, dyszoospermia and increased AI of germ cells.
研究实验性精索静脉曲张（EV）对大鼠同侧睾丸的影响。
通过部分结扎雄性SD大鼠的左肾静脉诱导建立EV模型，对照组大鼠进行假手术，6、12和18周后分别切除EV模型组和对照组大鼠的睾丸，评估左侧睾丸的约翰逊评分、生精小管超微结构、睾丸内睾酮浓度（ITC）和生殖细胞凋亡指数（AI）。
6周、12周和18周EV组的约翰逊评分分别为（6.92±0.52）、（4.83±0.41）和（2.95±0.26），ITC分别为（6.32±0.85）、（5.17±0.76）和（4.11±0.69），AI分别为（5.32±1.23）、（15.21±0.97）和（21.13±1.12），均显著低于相应对照组（9.56±0.35、9.63±0.31、9.39±0.46）、（9.64±1.23、9.38±0.69、9.73±0.49）和（3.21±1.15、3.43±1.21、3.61±1.15）（P<0.05），前两者随精索静脉曲张持续时间延长呈逐渐下降趋势，后者则显著升高。随着精索静脉曲张时间延长，生精小管超微结构损伤加重。
EV可导致ITC逐渐下降、精子发生障碍及生殖细胞AI升高。
