Langdale-Brown B, Haqqani M T
Dept. of Histopathology, Walton Hospital, Liverpool, U.K.
Scand J Gastroenterol. 1990 Feb;25(2):127-33. doi: 10.3109/00365529009107933.
Endoscopic gastric biopsy specimens from 230 consecutive patients in a North Liverpool District were histologically studied by routine light microscopy and ultraviolet fluorescence after acridine orange staining. Eighty patients with chronic gastritis were further studied with regard to type of gastritis and its activity, presence of Campylobacter pylori (CP), and degree of colonization of the gastric mucosa. Miscellaneous gastritis, gastric ulcers, erosions, neoplasms, and histologically normal specimens were excluded from the study. The results show statistically significant correlation between chronic gastritis and CP (P = 0.01, Mann-Whitney test). The activity of gastritis correlated well with CP, but there was no statistical significance between the density of neutrophils and degree of CP colonization (P greater than 0.5, Mann-Whitney test). In a small sample of CP-positive specimens acridine orange stain was compared with Warthin-Starry, Giemsa (modified), cresyl fast violet, and haematoxylin and eosin stains. Acridine orange stain in the histologic identification of CP has been used only once before in a large study.
对来自北利物浦地区的230例连续患者的内镜胃活检标本进行了组织学研究，采用常规光学显微镜和吖啶橙染色后的紫外线荧光观察。对80例慢性胃炎患者进一步研究了胃炎类型及其活动度、幽门弯曲菌（CP）的存在情况以及胃黏膜的定植程度。本研究排除了其他类型胃炎、胃溃疡、糜烂、肿瘤以及组织学正常的标本。结果显示慢性胃炎与CP之间存在统计学显著相关性（P = 0.01，Mann-Whitney检验）。胃炎活动度与CP密切相关，但中性粒细胞密度与CP定植程度之间无统计学意义（P大于0.5，Mann-Whitney检验）。在一小部分CP阳性标本中，将吖啶橙染色与Warthin-Starry染色、改良吉姆萨染色、甲酚固紫染色以及苏木精和伊红染色进行了比较。在一项大型研究中，吖啶橙染色在CP的组织学鉴定中仅被使用过一次。
