Mikkola Milla, Olsson Cia, Palgi Jaan, Ustinov Jarkko, Palomaki Tiina, Horelli-Kuitunen Nina, Knuutila Sakari, Lundin Karolina, Otonkoski Timo, Tuuri Timo
Program of Developmental and Reproductive Biology, Biomedicum Helsinki, PO Box 63, 00014 University of Helsinki, Helsinki, Finland.
BMC Dev Biol. 2006 Aug 8;6:40. doi: 10.1186/1471-213X-6-40.
Individual differences between human embryonic stem cell (hESC) lines are poorly understood. Here, we describe the derivation of five hESC lines (called FES 21, 22, 29, 30 and 61) from frozen-thawed human embryos and compare their individual differentiation characteristic.
The cell lines were cultured either on human or mouse feeder cells. The cells grew significantly faster and could be passaged enzymatically only on mouse feeders. However, this was found to lead to chromosomal instability after prolonged culture. All hESC lines expressed the established markers of pluripotent cells as well as several primordial germ cell (PGC) marker genes in a uniform manner. However, the cell lines showed distinct features in their spontaneous differentiation patterns. The embryoid body (EB) formation frequency of FES 30 cell line was significantly lower than that of other lines and cells within the EBs differentiated less readily. Likewise, teratomas derived from FES 30 cells were constantly cystic and showed only minor solid tissue formation with a monotonous differentiation pattern as compared with the other lines.
hESC lines may differ substantially in their differentiation properties although they appear similar in the undifferentiated state.
人们对人类胚胎干细胞（hESC）系之间的个体差异了解甚少。在此，我们描述了从冻融人类胚胎中获得的5个人类胚胎干细胞系（称为FES 21、22、29、30和61），并比较了它们各自的分化特征。
这些细胞系在人或小鼠饲养细胞上培养。细胞生长明显更快，并且仅在小鼠饲养细胞上才能通过酶解传代。然而，发现长时间培养后这会导致染色体不稳定。所有hESC系均以一致的方式表达已确立的多能细胞标志物以及几个原始生殖细胞（PGC）标志物基因。然而，这些细胞系在其自发分化模式中表现出明显特征。FES 30细胞系的胚状体（EB）形成频率明显低于其他细胞系，并且EB内的细胞分化较难。同样，与其他细胞系相比，源自FES 30细胞的畸胎瘤一直呈囊性，仅显示少量实体组织形成，且分化模式单一。
hESC系在分化特性上可能存在很大差异，尽管它们在未分化状态下看起来相似。
