Tanaka Y, Yano S, Okada K, Ishikawa T, Himeno M, Kato K
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Biochem Biophys Res Commun. 1990 Feb 14;166(3):1176-82. doi: 10.1016/0006-291x(90)90990-5.
Involvement of endosomes in transport of newly synthesized acid phosphatase to lysosomes was investigated using the Golgi fraction (GF1 + 2), enriched in endosomes. The Golgi fraction (GF1 + 2) was prepared from the livers of rats given [35S]methionine and asialofetuin conjugated-horseradish peroxidase (HRP). Newly synthesized acid phosphatase in the endosomes containing internalized asialofetuin-HRP was measured as a loss of the detectable labeled enzyme after 3,3'-diaminobenzidine (DAB) and H2O2 reaction, due to formation of insoluble polymers which reduce protein antigenicity. With this procedure, acid phosphatase was all but undetectable in the Golgi fraction. Thus, newly synthesized acid phosphatase is apparently transported to lysosomes by endosomes.
利用富含内体的高尔基体组分（GF1 + 2）研究了内体在新合成的酸性磷酸酶向溶酶体转运中的作用。高尔基体组分（GF1 + 2）是从给予[35S]甲硫氨酸和去唾液酸胎球蛋白偶联辣根过氧化物酶（HRP）的大鼠肝脏中制备的。通过3,3'-二氨基联苯胺（DAB）和H2O2反应后可检测的标记酶的损失来测量含有内化去唾液酸胎球蛋白-HRP的内体中新合成的酸性磷酸酶，这是由于形成了降低蛋白质抗原性的不溶性聚合物。通过该程序，在高尔基体组分中几乎检测不到酸性磷酸酶。因此，新合成的酸性磷酸酶显然是通过内体转运到溶酶体的。
