实验性肝纤维化中赖氨酰氧化酶的合成

Synthesis of lysyl oxidase in experimental hepatic fibrosis.

作者信息

Wakasaki H, Ooshima A

机构信息

Department of Pathology, Wakayama Medical College, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Feb 14;166(3):1201-4. doi: 10.1016/0006-291x(90)90993-w.

DOI:10.1016/0006-291x(90)90993-w

PMID:1689573

Abstract

The synthesis of lysyl oxidase, which initiates the cross-linking of collagen and elastin, was investigated in carbon tetrachloride (CCl4) induced fibrotic liver of rat. Lysyl oxidase activity of the fibrotic liver was 4 times greater than that of normal liver. mRNAs from the livers of normal and CCl4-treated rats were prepared for in vitro protein synthesis and the products were analyzed by immunoprecipitation with a monoclonal antibody against lysyl oxidase. The mRNAs from the fibrotic liver gave more than 3 times higher level of messenger copies for lysyl oxidase than did mRNAs from normal liver. The molecular weight of the nascent lysyl oxidase was 48,000.

摘要

在四氯化碳（CCl4）诱导的大鼠肝纤维化模型中，对启动胶原蛋白和弹性蛋白交联的赖氨酰氧化酶的合成进行了研究。纤维化肝脏中赖氨酰氧化酶的活性比正常肝脏高4倍。制备正常大鼠和CCl4处理大鼠肝脏的mRNA用于体外蛋白质合成，并使用抗赖氨酰氧化酶单克隆抗体通过免疫沉淀法分析产物。纤维化肝脏的mRNA产生的赖氨酰氧化酶信使拷贝水平比正常肝脏的mRNA高3倍以上。新生赖氨酰氧化酶的分子量为48,000。