Siegel R C, Chen K H, Greenspan J S, Aguiar J M
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2945-9. doi: 10.1073/pnas.75.6.2945.
Lysyl oxidase catalyzes the crosslinking of collagen and elastin. Lysyl oxidase activity was measured and localized in rat liver during the evolution of hepatic fibrosis induced by CCl4. Enzyme activity measured with DL-[6-3H]-lysine-labeled collagen substrates in liver and plasma increased sharply after approximately 3 wk of injection, reached a maximum at 6 wk, and then decreased. The increase in activity correlated histologically with early connective tissue septa formation, and the magnitude of increase was significantly greater than that found for the intracellular collagen biosynthetic enzymes protocollagen prolyl hydroxylase and lysyl hydroxylase. Indirect immunofluorescence studies showed that lysyl oxidase was present in association with collagen in the extracellular space. However, it was not possible to correlate the distribution pattern with a particular liver cell type. These observations suggest that serial measurements of lysyl oxidase activity in liver or plasma may be useful for correlating changes in connective tissue formation with histologic connective tissue deposition.
赖氨酰氧化酶催化胶原蛋白和弹性蛋白的交联。在四氯化碳诱导的大鼠肝纤维化演变过程中，对赖氨酰氧化酶活性进行了测定并定位。用DL-[6-³H]-赖氨酸标记的胶原蛋白底物测定的肝脏和血浆中的酶活性，在注射约3周后急剧增加，在6周时达到最大值，然后下降。活性增加在组织学上与早期结缔组织间隔形成相关，且增加幅度明显大于细胞内胶原蛋白生物合成酶原胶原脯氨酰羟化酶和赖氨酰羟化酶。间接免疫荧光研究表明，赖氨酰氧化酶存在于细胞外空间并与胶原蛋白相关。然而，无法将分布模式与特定的肝细胞类型相关联。这些观察结果表明，连续测量肝脏或血浆中的赖氨酰氧化酶活性可能有助于将结缔组织形成的变化与组织学结缔组织沉积相关联。
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