Bahnck Carolyn M, Fonseca Dina M
Genetics Program, Smithsonian Institution, Washington, DC 20008, USA.
Am J Trop Med Hyg. 2006 Aug;75(2):251-5.
A previously developed method to identify members of the Culex pipiens complex exploiting polymorphisms in a nuclear intron (acetylcholinesterase [ACE] based-assay) cannot differentiate the two forms of Cx. pipiens: form pipiens and form molestus. Notably, the two forms seem to differ extensively in behavior and physiology and likely have very different epidemiologic importance. Because they are morphologically indistinguishable, molecular methods are critical for the evaluation of their relative importance. Although the two forms of Cx. pipiens have been distinguished using a panel of microsatellite loci, such a protocol is laborious and expensive. We developed a rapid assay based on polymorphisms in the flanking region of a microsatellite locus. Used in conjunction with the ACE-assay, this new assay allows the identification of pure and hybrid populations of the two Cx. pipiens forms as well as those including Cx. quinquefasciatus. We discuss the usefulness of the method as well as limitations to its application.
一种先前开发的利用核内含子多态性（基于乙酰胆碱酯酶[ACE]的检测方法）来鉴定尖音库蚊复合体成员的方法，无法区分尖音库蚊的两种形态：尖音库蚊指名亚种和骚扰库蚊。值得注意的是，这两种形态在行为和生理上似乎有很大差异，并且可能具有非常不同的流行病学重要性。由于它们在形态上无法区分，分子方法对于评估它们的相对重要性至关重要。尽管已经使用一组微卫星位点区分了尖音库蚊的两种形态，但这样的方案既费力又昂贵。我们基于一个微卫星位点侧翼区域的多态性开发了一种快速检测方法。与ACE检测法结合使用，这种新检测方法能够鉴定出尖音库蚊两种形态的纯合群体和杂交群体，以及那些包含致倦库蚊的群体。我们讨论了该方法的实用性及其应用的局限性。
