Kojima Toshihiko, Nakahama Ken-ichi, Yamamoto Kiyotaka, Uematsu Hiroshi, Morita Ikuo
Department of Cellular Physiological Chemistry, Graduated School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Mol Cell Biochem. 2006 Dec;293(1-2):63-9. doi: 10.1007/s11010-006-2680-0. Epub 2006 Jul 31.
The changes in gene expression during senescence are very interesting. Early population doubling cDNA-1 (EPC-1, also known as pigment epithelial derived factor, PEDF) is one of the genes whose expression decreases dramatically during cellular aging. We examined whether or not EPC-1/PEDF promoter activity was affected by the cellular ageing using human diploid lung fibroblast cells in culture. Here we showed the promoter/enhancer region of EPC-1/PEDF existed at more than 1760 bp upstream from the transcriptional initiation site of the gene, and was regulated by both aging and cell cycle. These findings suggest that the expression of the EPC-1/PEDF gene is, at least in part, regulated transcriptionally in the cells. The analysis of the promoter region of the EPC-1/PEDF gene in this paper suggests the age- and cell cycle-dependent expression of specific transcriptional factor(s).
衰老过程中基因表达的变化非常有趣。早期群体倍增cDNA-1（EPC-1，也称为色素上皮衍生因子，PEDF）是其表达在细胞衰老过程中显著下降的基因之一。我们使用培养的人二倍体肺成纤维细胞研究了EPC-1/PEDF启动子活性是否受细胞衰老影响。在此我们表明，EPC-1/PEDF的启动子/增强子区域存在于该基因转录起始位点上游超过1760 bp处，并受衰老和细胞周期的调控。这些发现表明，EPC-1/PEDF基因的表达至少部分在细胞中受到转录调控。本文对EPC-1/PEDF基因启动子区域的分析表明存在特定转录因子的年龄和细胞周期依赖性表达。
