Gray M H, Rosenberg A E, Dickersin G R, Bhan A K
Department of Pathology, Massachusetts General Hospital, Boston 02130.
Hum Pathol. 1990 Feb;21(2):212-7. doi: 10.1016/0046-8177(90)90131-n.
Seven epithelioid and eight non-epithelioid vascular tumors were studied by the avidin-biotin-peroxidase method for the presence of endothelial- and epithelial-associated markers, using Ulex europaeus agglutinin-1 (UEA-1) lectin, and antibodies directed against factor VIII-related antigen, (FVIII-RA), vimentin, keratin, carcinoembryonic antigen, and epithelial membrane antigen. The cases included four epithelioid hemangiomas, two epithelioid hemangioendotheliomas (EHE), one epithelioid angiosarcoma (EAS), four common non-epithelioid capillary hemangiomas, and four non-epithelioid angiosarcomas. Staining for FVIII-RA, UEA-1, and vimentin were observed in all cases. The EAS showed staining for keratin in formalin-fixed, paraffin-embedded sections and in frozen sections. Staining for keratin was also observed in frozen sections of one EHE. Both keratin-positive vascular tumors were confirmed with electron microscopy. Carcinoembryonic antigen and epithelial membrane antigen stains were negative in all cases. Our results show that the epithelioid vascular tumors EHE and EAS, in addition to staining for the endothelial markers and vimentin, may also express the epithelial marker keratin. This is important since these tumors may closely resemble carcinomas by routine light microscopy. This study further underscores the importance of using a broad panel of immunohistochemical markers in the diagnostic workup of soft-tissue neoplasms.
采用抗生物素蛋白-生物素-过氧化物酶法，利用荆豆凝集素-1（UEA-1）凝集素以及针对因子VIII相关抗原（FVIII-RA）、波形蛋白、角蛋白、癌胚抗原和上皮膜抗原的抗体，对7例上皮样血管肿瘤和8例非上皮样血管肿瘤进行研究，以检测内皮细胞和上皮相关标志物的存在情况。这些病例包括4例上皮样血管瘤、2例上皮样血管内皮瘤（EHE）、1例上皮样血管肉瘤（EAS）、4例常见的非上皮样毛细血管瘤以及4例非上皮样血管肉瘤。所有病例均观察到FVIII-RA、UEA-1和波形蛋白染色阳性。在福尔马林固定、石蜡包埋切片以及冰冻切片中，EAS均显示角蛋白染色阳性。在1例EHE的冰冻切片中也观察到角蛋白染色阳性。这两种角蛋白阳性的血管肿瘤均经电子显微镜证实。所有病例的癌胚抗原和上皮膜抗原染色均为阴性。我们的结果表明，上皮样血管肿瘤EHE和EAS除了内皮标志物和波形蛋白染色阳性外，还可能表达上皮标志物角蛋白。这一点很重要，因为这些肿瘤在常规光学显微镜下可能与癌极为相似。本研究进一步强调了在软组织肿瘤诊断检查中使用多种免疫组织化学标志物的重要性。
