Lohr Christian, Bergstein Sandra, Hirnet Daniela
Abteilung für Allgemeine Zoologie, Fachbereich Biologie, TU Kaiserslautern, Postfach 3049, 67653 Kaiserslautern, Germany.
Cell Tissue Res. 2007 Jan;327(1):189-97. doi: 10.1007/s00441-006-0249-6. Epub 2006 Aug 1.
The antennal lobe (primary olfactory center of insects) is completely reorganized during metamorphosis. This reorganization is accompanied by changing patterns of calcium signaling in neurons and glial cells. In the present study, we investigated the developmental distribution of a major calcium-dependent protein, viz., calcium/calmodulin-dependent protein kinase II (CaM kinase II), in the antennal lobe of the sphinx moth Manduca sexta by using a monoclonal antibody. During synaptogenesis (developmental stages 6-10), we found a redistribution of CaM kinase II immunoreactivity, from a homogeneous distribution in the immature neuropil to an accumulation in the neuropil of the glomeruli. CaM kinase II immunoreactivity was less intense in olfactory receptor axons of the antennal nerve and antennal lobe glial cells. Western blot analysis revealed a growing content of CaM kinase II in antennal lobe tissue throughout metamorphosis. Injection of the CaM kinase inhibitor KN-93 into pupae resulted in a reduced number of antennal lobe glial cells migrating into the neuropil to form borders around glomeruli. The results suggest that CaM kinase II is involved in glial cell migration.
触角叶（昆虫的主要嗅觉中枢）在变态过程中会完全重组。这种重组伴随着神经元和神经胶质细胞中钙信号模式的变化。在本研究中，我们使用单克隆抗体研究了一种主要的钙依赖性蛋白，即钙/钙调蛋白依赖性蛋白激酶II（CaM激酶II）在烟草天蛾触角叶中的发育分布。在突触形成过程中（发育阶段6 - 10），我们发现CaM激酶II免疫反应性发生了重新分布，从不成熟神经纤维网中的均匀分布转变为在小球神经纤维网中的积累。在触角神经的嗅觉受体轴突和触角叶神经胶质细胞中，CaM激酶II免疫反应性较弱。蛋白质免疫印迹分析显示，在整个变态过程中，触角叶组织中CaM激酶II的含量不断增加。向蛹中注射CaM激酶抑制剂KN - 93会导致迁移到神经纤维网中以在小球周围形成边界的触角叶神经胶质细胞数量减少。结果表明，CaM激酶II参与神经胶质细胞迁移。
