Saeki Y, Chen J J, Shi L F, Okuda Y, Köhler H
IDEC Pharmaceuticals Corporation, La Jolla, CA 92037.
J Immunol. 1990 Mar 1;144(5):1625-8.
Previously we described a Th clone specific for a regulatory idiotype on 3A4, an anti-Id mAb that mimics a murine L1210/GZL tumor-associated Ag. In our studies, we determined the molecular target on the stimulating anti-Id antibody that is recognized by the Th clone. The Th clone responds with proliferation to the H chain of 3A4 but not to the L chain. Furthermore, the 3A4 chain stimulates this Th clone more efficiently than either the intact 3A4 or the Fab fragments, and the presentation by APC of the H chain is more resistant to chloroquine treatment than the presentation of the intact 3A4 molecule. These results suggest that regulatory T cells "see" their target idiotopes as linear sequence determinants present on isolated Ig chains, and show that this might have biologic advantages with respect to the mechanism of Ag presentation.
先前我们描述了一个针对3A4上调节性独特型的Th克隆，3A4是一种模拟鼠L1210/GZL肿瘤相关抗原的抗独特型单克隆抗体。在我们的研究中，我们确定了Th克隆所识别的刺激抗独特型抗体上的分子靶点。该Th克隆对3A4的重链有增殖反应，但对轻链无反应。此外，3A4重链比完整的3A4或Fab片段更有效地刺激该Th克隆，并且抗原呈递细胞对抗原重链的呈递比完整3A4分子的呈递对氯喹处理更具抗性。这些结果表明调节性T细胞将其靶独特型视为存在于分离的Ig链上的线性序列决定簇，并表明这在抗原呈递机制方面可能具有生物学优势。
