Webb S R, Sprent J
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
J Exp Med. 1990 Mar 1;171(3):953-8. doi: 10.1084/jem.171.3.953.
Contrary to existing dogma, evidence is presented that proliferative responses of mature unprimed T cells to Mlsa antigens involve CD8+ cells as well as CD4+ cells. The response of CD8+ cells to Mlsa antigens proved to be heavily dependent on help from CD4+ cells, and responses were stronger in three I-E+ strain combinations than in an I-E- combination. In I-E+ combinations, CD8+ blast cells accounted for 20-25% of the blasts generated from unseparated T cells responding to Mlsa-bearing stimulator cells in vitro; similar findings applied to blast cells generated in vivo. The observation that the majority (greater than or equal to 50%) of Mlsa-stimulated CD8+ cells (and CD4+ cells) were V beta 6+ indicated that CD8+ cells respond to Mlsa antigens, per se, rather than to nonspecific stimuli. Whether CD4+ and CD8+ cells use the same or different H-2-restricting elements to respond to Mlsa antigens has yet to be resolved.
与现有教条相反，有证据表明成熟未致敏T细胞对Mlsa抗原的增殖反应涉及CD8⁺细胞以及CD4⁺细胞。事实证明，CD8⁺细胞对Mlsa抗原的反应严重依赖于CD4⁺细胞的辅助，并且在三种I-E⁺品系组合中的反应比在I-E⁻组合中更强。在I-E⁺组合中，CD8⁺母细胞占体外对携带Mlsa的刺激细胞作出反应的未分离T细胞产生的母细胞的20%-25%；类似的发现也适用于体内产生的母细胞。大多数（大于或等于50%）受Mlsa刺激的CD8⁺细胞（和CD4⁺细胞）为Vβ6⁺这一观察结果表明，CD8⁺细胞本身对Mlsa抗原作出反应，而不是对非特异性刺激作出反应。CD4⁺和CD8⁺细胞是否使用相同或不同的H-2限制性元件来对Mlsa抗原作出反应还有待解决。
