Schmidt Martin, Gierlinger Notburga, Schade Ulrich, Rogge Tilmann, Grunze Michael
Angewandte Physikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany.
Biopolymers. 2006 Dec 5;83(5):546-55. doi: 10.1002/bip.20585.
We studied wood polymers in their native composite structure using mechanically isolated single spruce (Picea abies [L.] Karst.) fibers. Dichroic infrared spectra of fibers placed in a custom-built microfluidic cuvette were acquired in air, in liquid (heavy) water, and in liquid dimethylacetamide using a novel combination of synchrotron-based Fourier transform infrared microspectroscopy with polarization modulation. Differences were observed in the O-H stretching frequency region of the spruce spectra upon changing the ambient conditions. Analysis of these spectral variations provides information on hydrogen bonding, orientation, and accessibility of structural units of the wood polymers in the spruce cell walls. Our in situ approach contributes to a further understanding of the structural details of wood polymers in their native setting.
我们使用机械分离的单个云杉（Picea abies [L.] Karst.）纤维，研究了处于天然复合结构中的木材聚合物。利用基于同步加速器的傅里叶变换红外显微光谱与偏振调制的新型组合，在空气、液态（重）水和液态二甲基乙酰胺中，获取置于定制微流控比色皿中的纤维的二向色红外光谱。改变环境条件时，在云杉光谱的O-H伸缩频率区域观察到了差异。对这些光谱变化的分析提供了有关云杉细胞壁中木材聚合物结构单元的氢键、取向和可及性的信息。我们的原位方法有助于进一步了解天然环境中木材聚合物的结构细节。
