Department of Textile Materials and Design, Institute of Engineering Materials and Design, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia.
Carbohydr Res. 2010 Mar 30;345(5):621-30. doi: 10.1016/j.carres.2009.12.023. Epub 2009 Dec 29.
The cellulose-binding domain (CBD) is the second important and the most wide-spread element of cellulase structure involved in cellulose transformation with a great structural diversity and a range of adsorption behavior toward different types of cellulosic materials. The effect of the CBD from Clostridium cellulovorans on the supramolecular structure of three different sources of cellulose (cotton cellulose, spruce dissolving pulp, and cellulose linters) was studied. Fourier-transform infrared spectroscopy (FTIR) was used to record amides I and II absorption bands of cotton cellulose treated with CBD. Structural changes as weakening and splitting of the hydrogen bonds within the cellulose chains after CBD adsorption were observed. The decrease of relative crystallinity index of the treated celluloses was confirmed by FTIR spectroscopy and X-ray diffraction (XRD). X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) were used to confirm the binding of the CBD on the cellulose surface and the changing of the cellulose morphology.
纤维素结合域（CBD）是纤维素酶结构中第二个重要且广泛存在的元素，它与纤维素转化有关，具有很大的结构多样性和对不同类型纤维素材料的吸附行为范围。研究了来自纤维丁酸梭菌的 CBD 对三种不同来源的纤维素（棉纤维素、云杉溶解浆和纤维素绒）的超分子结构的影响。傅里叶变换红外光谱（FTIR）用于记录 CBD 处理的棉纤维素的酰胺 I 和 II 吸收带。在 CBD 吸附后，观察到纤维素链内氢键的弱化和分裂等结构变化。FTIR 光谱和 X 射线衍射（XRD）证实了处理过的纤维素的相对结晶度指数的降低。X 射线光电子能谱（XPS）和扫描电子显微镜（SEM）用于证实 CBD 在纤维素表面的结合以及纤维素形态的变化。
