Qiu Zhi-Feng, Li Tai-Sheng, Ruan Gui-Ren, Han Yang, Xie Jing, Zuo Ling-Yan, Li Yan-Ling, Wang Ai-Xia
Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Jun;28(3):386-90.
To study the dynamic changes of T lymphocyte subsets of AIDS patients during more than 24 months of highly active antiretrovirus therapy (HAART) with successful suppression of HIV replication and different CD4 + T cell restoration.
Totally 45 AIDS patients who had received HAART for more than 24 months were included. During HAART (including DO, M3, M6, M12, M18, and M24), the number of plasma HIV-1 RNA was measured quantitatively using the bDNA assay, and T lymphocyte subsets including CD3 + CD4 + cells, CD3 + CD8 + cells, naive CD4 + cells (CD4 + CD45RA + CD62L +), CD4 + CD28 + cells , and CD8 + CD38 + cells were detected with flow cytometer.
Among 45 patients, 24 patients (53.3%) whose plasma viral load decreased to less than 500 copies/ml at M6 and maintained to M24 were classified into three groups according to the CD4 + T cell count increments on M24 (compared with DO): group A (< 100/mm3), group B (100-200/mm3), and group C (> 200/mm3). After the initiation of HAART, T lymphocyte response, including CD4 + T cell counts, naive CD4 + cell counts, percentages of CD4 + CD28 + cells in these patients were improved gradually, while CD8 + CD38 + percentage decreased. The improvement of T lymphocyte response in group C was most remarkable even with highest plasma viral load and lowest CD4 T cell count on DO. Compared with group A and B, group C had significantly better improvement not only in the quantities of CD4 + T cell, but also in the CD28 + expression and naive CD4 + T cell populations.
T lymphocyte response of AIDS patients can be effectively reconstituted by HAART. Different dynamics of CD4 + CD28 + and naive CD4 + populations may considerably contribute to the quantity and cellular function restoration of CD4 + T lymphocyte.
研究获得性免疫缺陷综合征（AIDS）患者在接受高效抗逆转录病毒治疗（HAART）超过24个月期间，HIV复制得到成功抑制且CD4+T细胞恢复情况不同时T淋巴细胞亚群的动态变化。
纳入45例接受HAART超过24个月的AIDS患者。在HAART期间（包括第0个月、第3个月、第6个月、第12个月、第18个月和第24个月），采用分支DNA分析法对血浆HIV-1 RNA数量进行定量检测，并用流式细胞仪检测T淋巴细胞亚群，包括CD3+CD4+细胞、CD3+CD8+细胞、初始CD4+细胞（CD4+CD45RA+CD62L+）、CD4+CD28+细胞以及CD8+CD38+细胞。
45例患者中，24例（53.3%）在第6个月时血浆病毒载量降至低于500拷贝/ml并维持至第24个月，根据第24个月时CD4+T细胞计数的增加情况（与第0个月相比）分为三组：A组（<100/mm3）、B组（100 - 200/mm3）和C组（>200/mm3）。开始HAART后，这些患者的T淋巴细胞反应，包括CD4+T细胞计数、初始CD4+细胞计数、CD4+CD28+细胞百分比逐渐改善，而CD8+CD38+百分比下降。C组的T淋巴细胞反应改善最为显著，即使在第0个月时血浆病毒载量最高且CD4 T细胞计数最低。与A组和B组相比，C组不仅在CD4+T细胞数量上，而且在CD28+表达和初始CD4+T细胞群体方面的改善都明显更好。
HAART可有效重建AIDS患者的T淋巴细胞反应。CD4+CD28+和初始CD4+群体的不同动态变化可能对CD4+T淋巴细胞的数量和细胞功能恢复有很大贡献。
