Hamahashi Shugo, Onami Shuichi
Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Genome Inform. 2005;16(2):86-93.
The spindle orientation is a crucial piece of information to understand the development of embryo. The spindle forms during cell division and the cell divides along the spindle axis. Spindle orientation was measured in many different mutant embryos of Caenrohabditis elegans. However, the objectivity and the productivity of these measurements were low because these measurements were made manually. Here we present a system that automates the measurement of the spindle orientation in C. elegans embryo. Automation increases the objectivity and productivity of the measurement. We confirmed the applicability of the system by applying it to spindles during the second divisions in wild-type and mutant C. elegans embryos.
纺锤体定向是理解胚胎发育的关键信息。纺锤体在细胞分裂过程中形成，细胞沿着纺锤体轴进行分裂。在秀丽隐杆线虫的许多不同突变胚胎中测量了纺锤体定向。然而，这些测量的客观性和效率较低，因为它们是手动进行的。在此，我们展示了一个系统，该系统可自动测量秀丽隐杆线虫胚胎中的纺锤体定向。自动化提高了测量的客观性和效率。我们通过将该系统应用于野生型和突变型秀丽隐杆线虫胚胎第二次分裂期间的纺锤体，证实了该系统的适用性。
