Van Hoof Dennis, Mummery Christine L, Heck Albert J R, Krijgsveld Jeroen
Netherlands Institute of Developmental Biology, Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Expert Rev Proteomics. 2006 Aug;3(4):427-37. doi: 10.1586/14789450.3.4.427.
Human embryonic stem cells potentially represent an unlimited source of cells and tissues for regenerative medicine. Understanding signaling events that drive proliferation and specialization of these cells into various differentiated derivatives is of utmost importance for controlling their behavior in vitro. Major progress has been made in unraveling these signaling events with large-scale studies at the transcriptional level, but analysis of protein expression, interaction and modification has been more limited, since it requires different strategies. Recent advances in mass spectrometry-based proteomics indicate that proteome characterization can contribute significantly to our understanding of embryonic stem cell biology. In this article, we review mass spectrometry-based studies of human and mouse embryonic stem cells and their differentiated progeny, as well as studies of conditioned media that have been reported to support self-renewal of the undifferentiated cells in the absence of the more commonly used feeder cells. In addition, we make concise comparisons with related transcriptome profiling reports.
人类胚胎干细胞可能为再生医学提供无限的细胞和组织来源。了解驱动这些细胞增殖并分化为各种不同衍生物的信号事件，对于在体外控制它们的行为至关重要。在转录水平进行的大规模研究在揭示这些信号事件方面取得了重大进展，但蛋白质表达、相互作用和修饰的分析则较为有限，因为这需要不同的策略。基于质谱的蛋白质组学的最新进展表明，蛋白质组表征能够极大地促进我们对胚胎干细胞生物学的理解。在本文中，我们综述了基于质谱对人类和小鼠胚胎干细胞及其分化后代的研究，以及对条件培养基的研究，这些条件培养基据报道在没有更常用的饲养细胞的情况下能够支持未分化细胞的自我更新。此外，我们还与相关的转录组分析报告进行了简要比较。
