Molecular Endocrinology laboratory (KMEB), Medical Biotechnology Centre, University of South Denmark, Winslowparken 25, 1 sal, 5000 Odense, Denmark.
In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):259-68. doi: 10.1007/s11626-010-9281-7. Epub 2010 Mar 3.
The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk/).
人类胚胎干细胞的衍生和特性提供了一种具有临床应用潜力的多能干细胞来源。利用来自两家体外受精诊所的本地胚胎，我们衍生并鉴定了五个新的细胞系，用于非临床环境。对临床数据的分析表明，大多数胚胎（94.5%）未能达到囊胚发育阶段，而且无论胚胎的发育状态如何，需要 248 个胚胎才能创建一个干细胞系。从发育到囊胚阶段的胚胎数量（69 个）来看，只有 8.7%的胚胎发育成了细胞系。使用整个囊胚的外生体，我们在 2005 年至 2006 年间在丹麦欧登塞衍生了五个新的、未报道的细胞系。通过 RT-PCR、染色、核型分析、EB 形成和畸胎瘤形成进行了特征鉴定。KMEB hESC 系将来可通过英国干细胞库（http://www.ukstemcellbank.org.uk/）获得。
