Li Min, Fan Liu-Yin, Zhang Wei, Sun Ji, Cao Cheng-Xi
Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240 Shanghai, PR China.
J Pharm Biomed Anal. 2007 Jan 4;43(1):387-92. doi: 10.1016/j.jpba.2006.07.003. Epub 2006 Aug 9.
A capillary zone electrophoresis (CZE) method was developed for the quantitative analysis of lovastatin (Lvt) in capsule of monascus-Chinese medicine. Lvt in the capsule was separated using an electrolyte system consisting of 16% ethanol (v/v) in 60 mM Gly-sodium hydroxide buffer, pH 10.5, 16 kV applied voltage, 238 nm detection wavelength with a capillary of 51 cm x 75 microm i.d (43 cm to detector). Under the optimized conditions, the linear response of Lvt concentration ranges from 4.0 to 240 microg/mL with high correlation coefficient (r=0.9998, n=9), the limits of detection (LOD) and quantification (LOQ) for Lvt are 0.73 and 2.42 microg/mL, the precision values (expressed as R.S.D.) of intra-day and inter-day are 1.40-2.12% and 1.47-3.88%, respectively. The recoveries of the analyte at three concentration levels are 90.28-100.71%. The developed method can be well used for the quantification of Lvt in the drug in commercial formulations.
建立了一种毛细管区带电泳（CZE）法，用于定量分析中药红曲胶囊中的洛伐他汀（Lvt）。胶囊中的Lvt采用由60 mM甘氨酸 - 氢氧化钠缓冲液（pH 10.5）中含16%乙醇（v/v）组成的电解质体系进行分离，施加电压16 kV，检测波长238 nm，使用内径75 μm、总长51 cm（至检测器长度为43 cm）的毛细管。在优化条件下，Lvt浓度的线性响应范围为4.0至240 μg/mL，相关系数高（r = 0.9998，n = 9），Lvt的检测限（LOD）和定量限（LOQ）分别为0.73和2.42 μg/mL，日内和日间精密度值（以相对标准偏差R.S.D.表示）分别为1.40 - 2.12%和1.47 - 3.88%。在三个浓度水平下分析物的回收率为90.28 - 100.71%。所建立的方法可很好地用于商业制剂中该药物中Lvt的定量分析。
