Heyland Jan, Wiegandt Katharina, Goepfert Christiane, Nagel-Heyer Stefanie, Ilinich Eduard, Schumacher Udo, Pörtner Ralf
Faculty of Life Science, Hamburg University of Applied Science, Hamburg, Germany.
Biotechnol Lett. 2006 Oct;28(20):1641-8. doi: 10.1007/s10529-006-9144-1. Epub 2006 Aug 11.
Since articular cartilage is subjected to varying loads in vivo and undergoes cyclic hydrostatic pressure during periods of loading, it is hypothesized that mimicking these in vivo conditions can enhance synthesis of important matrix components during cultivation in vitro. Thus, the influence of intermittent loading during redifferentiation of chondrocytes in alginate beads, and during cartilage formation was investigated. A statistically significant increased synthesis of glycosaminoglycan and collagen type II during redifferentiation of chondrocytes embedded in alginate beads, as well as an increase in glycosaminoglycan content of tissue-engineered cartilage, was found compared to control without load. Immunohistological staining indicated qualitatively a high expression of collagen type II for both cases.
由于关节软骨在体内承受着不同的负荷，并且在负荷期间经历周期性流体静压，因此推测模拟这些体内条件可以在体外培养期间增强重要基质成分的合成。因此，研究了藻酸盐珠中软骨细胞再分化期间以及软骨形成期间间歇性负荷的影响。与无负荷的对照组相比，发现藻酸盐珠中包埋的软骨细胞再分化期间糖胺聚糖和II型胶原的合成有统计学意义的增加，以及组织工程软骨的糖胺聚糖含量增加。免疫组织化学染色定性显示两种情况下II型胶原的高表达。
