Zaidi Syed Faisal, Awale Suresh, Kalauni Surya Kant, Tezuka Yasuhiro, Esumi Hiroyasu, Kadota Shigetoshi
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Planta Med. 2006 Oct;72(13):1231-4. doi: 10.1055/s-2006-947189. Epub 2006 Aug 10.
In the course of our search for anticancer agents based on a novel anti-austerity strategy, we found that the 70 % EtOH extract of "Pini Resina" showed 100 % preferential cytotoxicity at the concentration of 50 microg/mL. Further bioassay-guided fractionation and purification led to the isolation of 15 compounds including one new compound 7-oxo-13 alpha-hydroxyabiet-8(14)-en-18-oic acid (1). Their structures were elucidated on the basis of spectroscopic analysis. Among the isolated compounds, methyl abieta-8,11,13-trien-18-oate (7) showed the most potent preferential cytotoxicity at 10 microg/mL under nutrient-deprived condition.
在我们基于一种新型抗紧缩策略寻找抗癌药物的过程中，我们发现“松脂”70%乙醇提取物在浓度为50微克/毫升时表现出100%的优先细胞毒性。进一步通过生物测定引导的分级分离和纯化，分离出15种化合物，其中包括一种新化合物7-氧代-13α-羟基枞-8(14)-烯-18-酸(1)。它们的结构通过光谱分析得以阐明。在分离出的化合物中，枞酸甲酯-8,11,13-三烯-18-酯(7)在营养缺乏条件下，于10微克/毫升时表现出最强的优先细胞毒性。
