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有限的N端序列分析

Limited N-terminal sequence analysis.

作者信息

Matsudaira P

出版信息

Methods Enzymol. 1990;182:602-13. doi: 10.1016/0076-6879(90)82047-6.

DOI:10.1016/0076-6879(90)82047-6
PMID:1690332
Abstract

Current methods in protein purification permit sequence analysis of picomole quantities of proteins and peptides. The key to obtaining sequence is to choose as the last step in the purification protocol the simplest (usually the fastest) method for isolating the protein or peptide.

摘要

目前蛋白质纯化方法能够对皮摩尔量的蛋白质和肽进行序列分析。获得序列的关键在于，在纯化方案的最后一步选择分离蛋白质或肽的最简单（通常也是最快）的方法。

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